However, the ′ protruding termini decreased(7 %),instead of additional ′ recessive ones(-,-,- ;7 0%).在Taq +PwoDNA聚合酶扩增的PCR产物末端中得到 ′端 +A的仅占 7 % ,而 - 的占 8% ,与前者显著不同 .
LGWV and LVWV were found to be C termini of proteins in the database.蛋白质数据库检索结果表明 ,有多个蛋白质具有 LGWV、 LVWV末端 ,没有检索到任何具有 DEWV末端的蛋白质 .
Methods Total RNA was isolated from dengue type virus 0 strain (D 0) infected C/ cells. With this RNA as templete, t方法 从D 0 感染的C / 细胞中提取总RNA ,以该RNA为模板 ,利用RACE法 ,分别扩增D 0 株的 ′和 ′末端cDNA片段。
Methods: sflt gene was cloned from the th day mouse embryos, the expression efficiency of sflt was compared with tha方法:通过RTPCR从胚胎小鼠中克隆可溶性VEGF受体基因sflt,并比较sflt与 ′末端缺失可溶性VEGF受体基因 ′Δflt在COS7细胞中的表达效率;
Results 8 YAC termini were isolated from YAC 77E, 9C, D8 and 8D.Conclusion The results suggest that inverse PCR be an 果用反向PCR方法，分别从77E、9C、D8和8D共个YAC分离得到各自的左右末端。 结论该方法简便、有效，适用于所有的YAC克隆