Methods Total RNA was isolated from dengue type virus 0 strain (D 0) infected C/ cells. With this RNA as templete, the cDNA of both ′ and ′ termini of D 0 were amplified using RACE method respectively.
Methods Total RNA was isolated from dengue type virus 0 strain (D 0) infected C/ cells. With this RNA as templete, the cDNA of both ′ and ′ termini of D 0 were amplified using RACE method respectively.
方法 从D 0 感染的C / 细胞中提取总RNA ,以该RNA为模板 ,利用RACE法 ,分别扩增D 0 株的 ′和 ′末端cDNA片段。