Results 8 YAC termini were isolated from YAC 77E, 9C, D8 and 8D.Conclusion The results suggest that inverse PCR be an efficient method for isolating YAC termini.
Results 8 YAC termini were isolated from YAC 77E, 9C, D8 and 8D.Conclusion The results suggest that inverse PCR be an efficient method for isolating YAC termini.
果用反向PCR方法，分别从77E、9C、D8和8D共个YAC分离得到各自的左右末端。 结论该方法简便、有效，适用于所有的YAC克隆