Three different Avian Influenza subtypes virus (H5N1,H6N2,H9N2) were vaccinated to chicken embryos,collected chorioallan将本实验室分离保存、已经过PCR扩增、核酸测序鉴定的三种不同亚型禽流感病毒(AIVH5N1、H6N2、H9N2)分别接种鸡胚,收集尿囊液,分离提取AIV总RNA。