Cryostat sections were studied with immunohistochemistry for glycogen synthase kinase (GSK ),cyclic dynamic kinase 行冰冻切片，做糖原合成激酶（GSK ）、细胞周期动态激酶（CDK）、蛋白磷酸酶（PP）、蛋白磷酸酶A（PPA）、微管蛋白（tubulin）免疫组化染色。