Dissolve the sample in the solution of ammonium chloride,use Eriochrome Black T as an indicator and titrate with EDTA to以％的氯化铵溶液溶解样品，用EBT作指示剂，EDTA滴定，测定（ZnCO ＋ZnO＋CaCO ＋MgCO ）含量。
Electrochemical study on interaction of eriochrome black T with bovine serum albumin电化学法研究蛋白质与铬黑T的相互作用