The optimum pH and temperature of total endopeptidases in crude extracts were pH 7.0,0 ℃. Besides,Ca+,Zn+ and cystine co黄瓜叶片粗提液总内肽酶的最适pH为7.0,最适温度为0℃,内肽酶活性可被Ca+、Zn+、半胱氨酸等激活。