The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition.
The fetal liver stem cells were isolated by collagenase digestion, gravity sedimentation and density gradient centrifugation, identified by immunocytochemistry and evaluated by flow cytometry for their proliferation condition.
采用胶原酶消化、重力沉降及密度梯度离心方法分离人胎肝干细胞，通过免疫细胞化学方法对其进行初步鉴定，以及应用流式细胞仪等对其生长状况进行评估。