Methods: The invasiveness w as assessed by penetration through a m atrigel-coated filter, the proliferation was determ ined by the m ethod reported by Gillies and cellsurface ICAM- and sICAM- analyzed by flow cytom etry and ELISA.
Methods: The invasiveness w as assessed by penetration through a m atrigel-coated filter, the proliferation was determ ined by the m ethod reported by Gillies and cellsurface ICAM- and sICAM- analyzed by flow cytom etry and ELISA.
方法：根据细胞穿过铺有Matrigel胶多孔膜数量检测细胞侵袭力，用改良龙胆紫吸收比色计数法检测细胞增殖，以流式细胞分析和酶联免疫吸附试验检测ICAM－、sICAM－表达水平。