The mRNA expression levels of titin and nebulin in the diaphragmatic muscles of rabbits in each group were detected by reverse transcription-polymerase chain reaction(RT-PCR).
The mRNA expression levels of titin and nebulin in the diaphragmatic muscles of rabbits in each group were detected by reverse transcription-polymerase chain reaction(RT-PCR).
采用逆转录-聚合酶链反应(RT-PCR)法检测膈肌组织中肌联蛋白(titin)和伴肌动蛋白(nebu lin)mRNA的表达。