A gene fragment encoding human monocyte chemoattratctant protein-1 (MCP-1) was subcloned into pEX31A. MS2/MCP-1 fusion protein was expressed in E. coli and accounted for about 15% of total cell proteins.
A gene fragment encoding human monocyte chemoattratctant protein-1 (MCP-1) was subcloned into pEX31A. MS2/MCP-1 fusion protein was expressed in E. coli and accounted for about 15% of total cell proteins.
将编码人单核细胞趋化蛋白－1（MCP－1）的基因亚克隆到大肠杆菌表达载体pEX31A中，在大肠杆菌中表达出MS2／MCP－1融合蛋0白，该表达产物约占菌体总蛋白的15％左右，Westernblot检测表明，表达产物可与MCP－1抗体特异反应。