Objective : To clone and express aroE gene which encodes shikimate dehydrogenase in Escherichia coli in order to improve the shikimate dehydrogenase product and confirm its new specific activity of quinate dehydrogenase.
Objective : To clone and express aroE gene which encodes shikimate dehydrogenase in Escherichia coli in order to improve the shikimate dehydrogenase product and confirm its new specific activity of quinate dehydrogenase.
目的:克隆表达大肠杆菌莽草酸脱氢酶基因aroE,并测定其生物学活性。