5.5. The cell was incubated at 280C with 5.0% seed culture on ashaker operated at l7Orlmin, L-Tyr was added until 0.5 g L at 1 5hand the cell was collected after 21 h, the enzyme specific activity ashigh as 19.0 mU ? mg~?
5.5. The cell was incubated at 280C with 5.0% seed culture on ashaker operated at l7Orlmin, L-Tyr was added until 0.5 g L at 1 5hand the cell was collected after 21 h, the enzyme specific activity ashigh as 19.0 mU ? mg~?
以5％接种量接种，于170r/min，28℃培养15h后添加L-酪氨酸至终浓度0.5g/L，继续培养至21h收集菌体，获得最大酶比活力达19.0mU/mg。