Calvarial bones were taken out from newborn Wistar rats;osteoblast cells were obtained via enzymatic digestion method.
Calvarial bones were taken out from newborn Wistar rats;osteoblast cells were obtained via enzymatic digestion method.
取出生24 h内的Wistar大鼠的颅骨,以酶消化法获得成骨细胞,将传至第3代的细胞冻存、复苏,对细胞进行生物学特性观察,研究了冻存复苏后的大鼠颅骨成骨细胞的生物学特性。