Method: The Thermo ODS C8 column(0 mm×. mm,μm) was used, the mobile phase was methanol -0.% phosphorie acid solution(8: ) , the flow rate was .0 ml·min- and the detection wavelength was at nm.
Method: The Thermo ODS C8 column(0 mm×. mm,μm) was used, the mobile phase was methanol -0.% phosphorie acid solution(8: ) , the flow rate was .0 ml·min- and the detection wavelength was at nm.
方法:色谱柱为Thermo ODS C8柱(0 mm×． mm,μm),流动相为甲醇-0．％磷酸溶液(8:),流速为．0 ml·min-,检测波长为 nm。 结果:大黄素在0．0-0．9μg(r =0.999 );