METHODS: HepG_ cells were divided into control group, damage group and protective group, then were added with different concentrations of H_O_ and lipoic acid. Two hours later, the single cell gel electrophoresis (SCGE) was used to detect the DNA damage.
METHODS: HepG_ cells were divided into control group, damage group and protective group, then were added with different concentrations of H_O_ and lipoic acid. Two hours later, the single cell gel electrophoresis (SCGE) was used to detect the DNA damage.
方法:将细胞分为对照组、损伤组和保护组,依次加入不同浓度的HO及硫辛酸,应用SCGE检测HO引起细胞DNA损伤.