(3) Hyp was purified after cell collection, suspension, ammonium sulfate precipitation and Ni-NTA column chromatography.
(3) Hyp was purified after cell collection, suspension, ammonium sulfate precipitation and Ni-NTA column chromatography.
3、离心发酵液以收集菌体,用缓冲液悬浮菌体,经过超声破碎、硫酸铵分级沉淀和Ni-NTA亲和层析,得到了电泳纯的Hyp。