METHODS: Frozen sections of the gamasid mites 0 days and more than 00 days after biting suckling mice inoculated with HFRSV were in situ hybridized with dig labelled HFRSV cDNA probes .
METHODS: Frozen sections of the gamasid mites 0 days and more than 00 days after biting suckling mice inoculated with HFRSV were in situ hybridized with dig labelled HFRSV cDNA probes .
方法：用上海真厉螨与柏氏禽刺螨叮咬HFRSV接种乳小鼠，取叮咬后d0及d00以上的上海真厉螨与柏氏禽刺螨冰冻切片，用地高辛标记HFRSVcDNA核酸探针原位分子杂交检测其体内病毒RNA。