It showed BL21(DE3) was the better bacterial host. The method of pH-stat fed-batch culture was applied to obtain high cell density. The final cell density OD600 of grown pNS2/BL21(DE3) was 71.64 and the volumetric productivity was 0.8g/L.
It showed BL21(DE3) was the better bacterial host. The method of pH-stat fed-batch culture was applied to obtain high cell density. The final cell density OD600 of grown pNS2/BL21(DE3) was 71.64 and the volumetric productivity was 0.8g/L.
研究了重组质粒pNS2在两种宿主菌中的摇瓶与10L发酵罐培养表达情况，确定了较适的宿主菌为BL21(DE3)，采用pH-stat补料分批发酵，工程菌pNS2／BL21(DE3)最终菌体密度OD_(600)达71.64，表达量为0.8g／L。