Trepan was drilled the culture sheets coated with HAEC to makequantified models of damaged HAEC,on which the lacks were thencovered with fibrin clot. Subsequently,different concentration of EGF, bFGFand TGF-β were added into the sheets respectively.
Trepan was drilled the culture sheets coated with HAEC to makequantified models of damaged HAEC,on which the lacks were thencovered with fibrin clot. Subsequently,different concentration of EGF, bFGFand TGF-β were added into the sheets respectively.
环钻钻切 HAEC 培养板,建立 HAEC 定量损伤模型,用制备的纤维蛋白块覆盖环钻钻切范围,分别加入不同浓度的 EGF、bFGF和 TGF-β,Giemsa 染色倒置显微镜观察 HAEC 生长移行情况,-溴脱氧尿苷(BrdU)掺入法检测 HAEC 的增殖效应。