Apoptosis index myocardium was detected by Tunel method and protein expression of Bcl-2, Bax, Cyt-c and caspase-3 genes were investigated by spimmunohistochemial stain.
Apoptosis index myocardium was detected by Tunel method and protein expression of Bcl-2, Bax, Cyt-c and caspase-3 genes were investigated by spimmunohistochemial stain.
采用缺口末端标记法(TUNEL)以及免疫组化法,检测心肌细胞凋亡千分率、Bcl-2、Bax、Cyt-c和caspase-3基因蛋白表达。