Methods GHcDNA was cleaved by two kinds of restriction enzyme from pUC9-GHcDNA and inserted into eukaryotic expression vector pcDNA ,froming recombinant pcDNA -GH plastid. Its sequence was identified by cleaving of enzyme and sequencing.
Methods GHcDNA was cleaved by two kinds of restriction enzyme from pUC9-GHcDNA and inserted into eukaryotic expression vector pcDNA ,froming recombinant pcDNA -GH plastid. Its sequence was identified by cleaving of enzyme and sequencing.
方法 用双酶切从 pUC9-GHcDNA中克隆出生长激素基因 ,然后定向插入真核细胞表达载体pcDNA 中 ,行酶切和测序以鉴定。