Methods Early somite stage (GD 8.) mouse embryos were explanted and cultured in rat serum medium (immediately centrifugal serum,ICS) with atRA(0,0.0,0.,0.,0.,.0,0.0 μmol/L) for 8 h to evaluate the embryo development and morphological differentiation.
Methods Early somite stage (GD 8.) mouse embryos were explanted and cultured in rat serum medium (immediately centrifugal serum,ICS) with atRA(0,0.0,0.,0.,0.,.0,0.0 μmol/L) for 8 h to evaluate the embryo development and morphological differentiation.
方法孕8.日龄小鼠胚胎移植于atRA终浓度为0、0.0、0.、0.、0.、.0、0.0μmol/L的全胚胎培养基中,体外连续培养8h,观察atRA对胚胎发育和器官形态分化的影响。