Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%.
Total RNA was prepared from immature Beijing wild soybean seeds by SDS-phenol method, and the total mRNA was purified by oligo(dT) cellulose affinity chromatography. The ratio of mRNA to total RNA was about 5.3%.
以SDS—苯酚法从北京野生大豆未成熟种子中制备总核糖核酸(RNA),经Oligo(dT)—纤维素柱亲和层析获得总信使核糖核酸(mRNA),约占总RNA的5.3％。