Approximately 3.5 kb hTERT gene was cut by EcoRⅠ and BamHⅠ from the retroviral vector pBABE-hTERT-puro and then inserted to the corresponding restriction enzyme sites of pBABE-puro-lox to make a new vector phTERTPlox.
Approximately 3.5 kb hTERT gene was cut by EcoRⅠ and BamHⅠ from the retroviral vector pBABE-hTERT-puro and then inserted to the corresponding restriction enzyme sites of pBABE-puro-lox to make a new vector phTERTPlox.
用EcoRⅠ和BamHⅠ酶切下逆转录病毒载体pBABE-hTERT-puro上约3.5kbhTERT基因片段,插入pBABE-puro-lox载体的相应酶切位点之间,重组的新载体称为phTERTPlox;