[Methods] Linearized pBABE-puro was cut by restriction enzyme NheⅠand ligated with loxP double strands to construct a vector pBABE-puro-lox.
[Methods] Linearized pBABE-puro was cut by restriction enzyme NheⅠand ligated with loxP double strands to construct a vector pBABE-puro-lox.
方法用NheⅠ酶切线性化pBABE-puro产生两个相同的黏端,与loxP双链混合连接构建成载体pBABE-puro-lox;