According to the predicted P0 sequences from the genome of Bombyx mori,a pair of primers were designed and a novel gene named CYPB9 of cytochrome P0 was successfully cloned from the midgut mRNA of Bombyx mori by RT-PCR(GenBank accession number: DQ ).
According to the predicted P0 sequences from the genome of Bombyx mori,a pair of primers were designed and a novel gene named CYPB9 of cytochrome P0 was successfully cloned from the midgut mRNA of Bombyx mori by RT-PCR(GenBank accession number: DQ ).
依据家蚕基因组P0预测序列设计对引物,以家蚕中肠为材料提取mRNA,采用RT-PCR方法克隆了P0家族基因CYPB9(GenBank登录号:DQ )。