In determining the optimal concentration of each antibody, we have used a strategy in which the capture antibody is first immobilized at a particular density on the predetermined site of membrane, and the labeled antibody is then varied to retain a maximu
In determining the optimal concentration of each antibody, we have used a strategy in which the capture antibody is first immobilized at a particular density on the predetermined site of membrane, and the labeled antibody is then varied to retain a maximu
在测定每个抗体的最佳浓度中,我们用到了这样一个方案:先将捕获抗体以特定的浓度固定在膜上预留的部位,然后改变标记抗体浓度得到最大的信号-噪音比例。