DNA restriction fragments or cloned vectors of five oncogenes (abl, myc, myb, fos, Ki-ras)and of IL-, IL- receptor, T-cell receptor β-chain and transferrin receptor were dotted onto nitrocellulose filters.
DNA restriction fragments or cloned vectors of five oncogenes (abl, myc, myb, fos, Ki-ras)and of IL-, IL- receptor, T-cell receptor β-chain and transferrin receptor were dotted onto nitrocellulose filters.
mRNA,以之为模板通过逆转录法得到以α-~ P标记的第一链cDNA,再以cDNA为探针同abl,myc,myb,fos,ras五种癌基因及TcR-β,IL-,IL-R,TfR四种与细胞生长增殖密切相关的基因片段进行斑点杂交。