RT PCR was used to amplify the ERβ exon wild type mRNA (ERβ WT) and spicing variant mRNA (ERβ ESV). Electrophoresis of PCR product was performed and the ratio of ERβ ESV / ERβ WT was calculated.
RT PCR was used to amplify the ERβ exon wild type mRNA (ERβ WT) and spicing variant mRNA (ERβ ESV). Electrophoresis of PCR product was performed and the ratio of ERβ ESV / ERβ WT was calculated.
将PCR产物进行电泳分析并测定ERβESV和ERβWT条带的累积光密度 ,计算ERβESV /ERβWT之值。