It was digested byBsl I enzyme after purification. Enzyme cutting products was evaluated by running through % agarose gel electrophoresis. Pro 87Leu mutationof PTPB gene were determined by 0.%bromium/ infrared.
It was digested byBsl I enzyme after purification. Enzyme cutting products was evaluated by running through % agarose gel electrophoresis. Pro 87Leu mutationof PTPB gene were determined by 0.%bromium/ infrared.
应用PCR扩增目的片段,再用Bsl I酶切, %琼脂糖凝胶电泳,0.%溴乙锭/紫外线检测上海地区中国人P 87L突变情况。