3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
3.The transcription of MC148 gene inserted into Ad-MC148 was confirmed by RT-PCR and protein SDS-PAGE .
经测定病毒滴度高达3．56X 10＼PFU／ml卜三、经RT－PCR和病毒转染细胞上清蛋白提纯，证明腺病毒重组体Ad－MC 48中有MC148基因并且可转录及翻译表达。