Results The IFN-γ expression yield was 40 4% of bacterial soluble proteins. The purification of the inclusion bodise was 70%. The total biological activity was 1 27×10 9 U.The specific activity was 1 31×10 9 U/L.
Results The IFN-γ expression yield was 40 4% of bacterial soluble proteins. The purification of the inclusion bodise was 70%. The total biological activity was 1 27×10 9 U.The specific activity was 1 31×10 9 U/L.
结果 IFN -γcDNA表达水平占菌体可溶性蛋白的 40 4% ,包涵体纯度为 70 % ,总生物活性为 1 2 7× 10 9U ,比活性达 1.31× 10 9U/L蛋白 ,活性回收率为 77.7%。