The gene disruption cassette combined the het- erologous dominant kanr resistance marker with a Cre/loxP-mediated marker removal procedure.
The gene disruption cassette combined the het- erologous dominant kanr resistance marker with a Cre/loxP-mediated marker removal procedure.
重组质粒pSH-CUP的构建,不仅解决了酵母转化子筛选标记问题和非酵母基因的引入,而且使LoxP-kanMX-loxP基因敲除体系在进行真核生物基因敲除时更加方便可行。