When the DO was controlled above 30%, carbon and nitrogen source was feeded at log phase,the dry cell weight and the expression level of reCPI-GST reached 26.2 g/L and 24.8% of the total cell protein, respectively.
When the DO was controlled above 30%, carbon and nitrogen source was feeded at log phase,the dry cell weight and the expression level of reCPI-GST reached 26.2 g/L and 24.8% of the total cell protein, respectively.
在3.7 L台式搅拌发酵罐中,控制溶氧在30%以上,重组菌的对数生长中期流加碳源和氮源,最终菌体光密度A600达到52.4,菌体干重为26.2g/L,目的蛋白的表达量达到24.8%。