The eluent is changed in a regular manner starting with a nonpolar solvent and gradually replacing it by a more polar one.
The eluent is changed in a regular manner starting with a nonpolar solvent and gradually replacing it by a more polar one.
洗脱液以常规的顺序更换，首先用非极性溶剂，然后逐步被极性较强的所替换。