A proteinase K digestion and phenol-chloroform extraction method was used to extract IBDV dsRNA from bursae of infected chicken. The VP gene was amplified by RT-PCR, and then cloned into pMD8-T vector for sequence analysis.
A proteinase K digestion and phenol-chloroform extraction method was used to extract IBDV dsRNA from bursae of infected chicken. The VP gene was amplified by RT-PCR, and then cloned into pMD8-T vector for sequence analysis.
用蛋白酶K消化、酚-氯仿抽提法成功提取出IBDV基因组RNA，用RT-PCR技术扩增出IBDV的结构蛋白基因VP，并将其克隆到pMD8-T载体。