3. Under optimum inducement condition (37℃, 0.3 mmol/L IPTG, 4 h), the content of recombinant protein could reach to 19% of total cell protein tested by Gene Genius Bio Imaging System.
3. Under optimum inducement condition (37℃, 0.3 mmol/L IPTG, 4 h), the content of recombinant protein could reach to 19% of total cell protein tested by Gene Genius Bio Imaging System.
3.最佳诱导条件为:0.3 mmol/L IPTG 37℃诱导4 h,表达量约占菌体总蛋白的19%。