The molecular mass of the enzyme subunit was about 37.5×103. The optimum pH and temperature for reduction were 6.0 and 45 ℃, respectively.
The molecular mass of the enzyme subunit was about 37.5×103. The optimum pH and temperature for reduction were 6.0 and 45 ℃, respectively.
粗酶经乙醇沉淀、Blue Sepharose Fast Flow亲和层析、DEAE Sepharose离子交换层析后在SDS-PAGE上显示为单一条带,其酶蛋白的亚基分子量(Mr)为37.5×103.该酶还原反应的最适pH为6.0,最适温度为45℃,是一个含Zn2+金属酶.