The conditions of the affinity chromatogram of p-Aminobenzamidine-SepharoseB were that: starting buffer is 0.M PB buffer (pH7.0), eluting buffer is 0.M PB-0.M NaCl solution (pH7.0).
The conditions of the affinity chromatogram of p-Aminobenzamidine-SepharoseB were that: starting buffer is 0.M PB buffer (pH7.0), eluting buffer is 0.M PB-0.M NaCl solution (pH7.0).
利用亲和层析技术做进一步纯化,采用对-氦基苯甲脒-SepharoseB亲和层析方法,纯化工艺条件为:上样液为0.M PB pH7.0,沈脱液为0.M PB-0.M NaCl pH7.0溶液。