Results The quantity of plasmid DNA isolated by the improved method was 3.2μg per milliliter culture of transformed bacteria,and the ratio of A260/280 was 1.91.The plasmid DNA could be fully digested by restriction enzyme.
Results The quantity of plasmid DNA isolated by the improved method was 3.2μg per milliliter culture of transformed bacteria,and the ratio of A260/280 was 1.91.The plasmid DNA could be fully digested by restriction enzyme.
结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91; 内切酶酶切完全;