HMGB and EGFP were subcloned into hytohemagglutinin (HA) tagged vector pcDNA HA by two steps, the construct of which was then transfected into 9 A cells and observed with fluorescence microscope.
HMGB and EGFP were subcloned into hytohemagglutinin (HA) tagged vector pcDNA HA by two steps, the construct of which was then transfected into 9 A cells and observed with fluorescence microscope.
通过两步亚克隆的方法,将HMGB和EGFP的编码序列以融合表达形式克隆到带有血凝素(hytohemagglutinin,HA)标记的载体pcDNA HA上,随后转染9 A细胞,在荧光显微镜下观察结果。