The effect of different concentrations of template DNA,primer,dNTPs and Mg2+on the PCR reaction was analyzed and the optimized RAPD and ISSR-PCR systems in radish were established.
The effect of different concentrations of template DNA,primer,dNTPs and Mg2+on the PCR reaction was analyzed and the optimized RAPD and ISSR-PCR systems in radish were established.
对影响PCR反应的主要因子———模板DNA、引物、dNTPs和Mg2+浓度进行优化,建立起适合于萝卜基因组DNA的RAPD和ISSR反应体系。