METHOD Human umbilicus vein endothelial cell and lipopolysaccharide were used to establish apoptosis cell models,and flow cytometer and confocal microscope were used to observe the effect of the medicated serum on ICAM-1 expression and intracellular Ca2+.
METHOD Human umbilicus vein endothelial cell and lipopolysaccharide were used to establish apoptosis cell models,and flow cytometer and confocal microscope were used to observe the effect of the medicated serum on ICAM-1 expression and intracellular Ca2+.
方法取人脐静脉内皮细胞(HUVEC),用脂多糖造成凋亡细胞模型,通过流式细胞仪、激光共聚焦显微镜观察丹参药物血清对ICAM-1表达及胞内Ca2+的影响。