MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D.
MethodsSome reaction condition was optimized by amplifying technology and method of PCR based on isolating genomic DNA from D.
方法在提取纯化美花石斛基因组DNA的基础上,利用PCR扩增技术和方法,对Mg2+,dNTP,模板DNA,引物,Taq聚合酶等反应条件进行优化。