hSel P137m was split by hydroxylamine and the result was analyzed by SDS-PAGE and Western blotting.
hSel P137m was split by hydroxylamine and the result was analyzed by SDS-PAGE and Western blotting.
方法: 利用生物信息学技术分析hSelP280m,hSelP137m的活性功能域,用羟胺切割hSelP137m,并用SDSPAGE及蛋白质印迹加以分析验证。