Construction and expression of prokaryotic expression vector of Cp The recombinant plasmids pET-28a-15,pET-28a-15/60 and pET-28a-23 were constructed by cloning CP15,CP15/60 and CP23 genes into prokaryotic expression vector pET-28a(+) and expressed in E.
Construction and expression of prokaryotic expression vector of Cp The recombinant plasmids pET-28a-15,pET-28a-15/60 and pET-28a-23 were constructed by cloning CP15,CP15/60 and CP23 genes into prokaryotic expression vector pET-28a(+) and expressed in E.
原核表达载体的构建及表达 将3个基因片段克隆到pET－28of＋）载体上构建成3个原核表达载体：pET－28a－15、pET－28al5／60和pET－28a－23，经酶切鉴定后在宿主菌BLZI（DE3）中表达。