In order to obtain artificial antigen of neomycin, neomycin was linked to Bovine Serum albumin(BSA) and keyhole limpet hemocyanin(KLH) respectively by EDC coupling. The conjugates BSA-NEO and KLH-NEO were identified by infrared spectrum.
In order to obtain artificial antigen of neomycin, neomycin was linked to Bovine Serum albumin(BSA) and keyhole limpet hemocyanin(KLH) respectively by EDC coupling. The conjugates BSA-NEO and KLH-NEO were identified by infrared spectrum.
以碳化二亚胺作为偶联剂,分别将新霉素(Neomycin,NEO)和牛血清白蛋白(Bovine serum albu-min,BSA)、血蓝蛋白(Keyhole limpet hemocyanin,KLH)进行偶联,得到偶联产物BSA-NEO和KLH-NEO。