Soybean Kunitz-type trypsin inhibitors are important antinutritious factors. The full-length DNA fragment of soybean Kunitz-type Trypsin Inhibitor gene KSTI3 was amplified by PCR technique,then PCR products were cloned into pMD18-T vector.
Soybean Kunitz-type trypsin inhibitors are important antinutritious factors. The full-length DNA fragment of soybean Kunitz-type Trypsin Inhibitor gene KSTI3 was amplified by PCR technique,then PCR products were cloned into pMD18-T vector.
以大豆基因组DNA为模板,利用聚合酶链式反应(PCR)技术克隆了大豆胰蛋白酶抑制剂基因KSTI3的全长DNA片段,并将其构建到pMD18-T vector上。