Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ.
Methods CD8 gene was cut out from plasmid pCDM8 by restriction endonuclease Xho Ⅰ. It was inserted into eukaryotic expression vector pCDNA and identified by restriction enzyme BamH Ⅰ.
方法 用限制性内切酶XhoⅠ酶切质粒pCDM8得到CD8基因 ,并将其插入pCDNA ,用BamHⅠ酶切鉴定。